Chromatography of steroids

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Paper Chromatography of Steroids

In Paper I of this series (1) a method was described for the paper chromatographic analysis of certain ketonic steroids as hydrazones formed with Girard’s Reagent T. When that technique was applied to the adrenal cortical hormones, however, certain deficiencies appeared. (a) The RI values of the individual steroid-Reagent T hydrazones were not sufficiently different to permit identification. (b...

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The Chromatography of Steroids

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Paper chromatography of C19 steroids.

The successful use of paper chromatography for t.he separation of corticoids (2) suggested the general usefulness of this procedure for relat,ed compounds. Two procedures for t.he separation of cardiac glycosides and aglycones have been described (3, 1). Preliminary reports (5, G) and a reference (7) have been made on methods for other steroid hormones. This paper describes an effective system ...

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Quantitative paper chromatography of reducing steroids of the adrenal cortex.

T. MANN AND E. LEONE I953 Neuberger, A. & Webster, T. A. (1946). Biochem. J. 40, 576. Newton, E. B., Benedict, S. R. & Dakin, H. D. (1926). Science, 64, 602. Pauly, H. (1904). Hoppe-Seyl. Z. 42, 508. Pirie, N. WV. (1933a). Biochem. J. 27, 202. Pirie, N. W. (1933 b). Biochem. J. 27, 1181. Potter, Van R. & Franke, K. W. (1935). J. Nutr. 9, 1. Roe, J. H. & Kuether, C. A. (1943). J. biol. Chem. 147...

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ژورنال

عنوان ژورنال: Journal of Chromatography A

سال: 1976

ISSN: 0021-9673

DOI: 10.1016/s0021-9673(00)99265-3